The isolated microalga Chlorella sorokiniana BENHA721_ABO4 was ‎grown in Bold’s basal medium (BBM) as a control,‎‏ ‏municipal ‎wastewater (WW), and wastewater enriched with BBM elements (WW+). ‎Cultivation in WW+ showed the highest‏ ‏cell number which represented ‎‎25.3 and 47.3% over that grown in WW and BBM, respectively. ‎However, rapid growth in‏ ‏WW+ was accompanied by significant ‎reduction in lipid content. Due to lipid accumulation in WW, it showed ‎the maximum‏ ‏significant lipid productivity of 16.2 mg L−1 day−1. ‎Microalgae cultivation in WW for 10 days showed 74.2, 83.3, and ‎‎78.0%‎‏ ‏removal efficiency for NO3–‎‏ ‏N, NH3–‎‏ ‏N and TP, respectively. In ‎addition, growth in WW significantly reduced polyunsaturated‏ ‏fatty ‎acids by 36.0% with respect to BBM in favor of monounsaturated fatty ‎acids. The present results confirmed that‏ ‏C. sorokiniana isolate ‎BENHA721_ABO4 grown in secondary effluent municipal wastewater ‎offers real potential for future‏ ‏application in wastewater treatment and ‎biodiesel production.‎