Amelioration of culture conditions of Mesenchymal Stem Cells (MSCs) and optimization of preconditioned micro-environments can increase the numbers of cultured and expanded of MSCs in vitro then used for therapeutic applications. Cell proliferation and survival rates are the main hurdles in the clinical use of MSCs. Cultivated MSCs Survival and proliferation are impaired by oxidative stress and pre-apoptotic factors released during cultivation processes. Hence, strategies that reduce cell death and elevates the viability and proliferation of MSCs will be added values for using MSCs in therapeutic uses. The present study discussed the efficiency of resveratrol (RSV) to ameliorate the survival and proliferative capacity of MSCs when used in the preconditioned medium of MSCs preparation procedures. It was revealed that MSCs pretreated with 10 μM of RSV in vitro exhibited a significant proliferation than cells not treated with RSV. RSV suppressed the apoptotic condition, stress of oxidation and cell death probabilities through its potential effect on cytoprotective gene Bcl-2 in addition regulating Bcl-2/Bax ratio.