أعضاء هيئة التدريس - جامعة بنها
Hematological consequences of polyethylene microplastics toxicity in male rats: Oxidative stress, genetic, and epigenetic links
Toxicology • 2023
معلومات البحث
المؤلفون
Amina A. Farag a, Heba S. Youssef b, Rania E. Sliem c, Walaa Bayoumie El Gazzar d,e,
Nashwa Nabil f, Maha M. Mokhtar a, Yasmin M. Marei e, Nesma S. Ismail a,
Shaimaa E. Radwaan c, Amira M. Badr g,h, Alaa El-Din Hamid Sayed i,j,*
الكلمات المفتاحية
Polyethylene microplastics
Hematological
Red blood cells
Genotoxicity
DNA methylation
Epigenecity
المجلة العلمية
Toxicology
الناشر
Not Available
المجلد
Not Available
العدد
Not Available
الصفحات
Not Available
publication.type
International
رابط البحث
Not Available
المواد المرفقة
Not Available
الملخص
There have been concerns about the potential health risks posed by microplastics (MP).
The detection of MP in a variety of food products revealed that humans are ingesting MP. Nevertheless,
there is a paucity of data about their impacts, as well as their uptake, on intestinal barrier
integrity. This study examined the toxic effects of oral administration of two doses of polyethylene
microplastics (PE-MP) (3.75 or 15 mg/kg/day for 5 weeks; mean particle size: 4.0–6.0 m) on the
intestinal barrier integrity in rats. Moreover, the effect of melatonin treatment with MP exposure
was also assessed. The PE-MP particle uptake, histopathological changes, Alcian blue staining,
Muc2 mRNA, proinflammatory cytokines (IL-1 and TNF- ), and cleaved caspase-3, as well as tight
junction proteins (claudin-1, myosin light-chain kinase (MLCK), occludin, and zonula occludens-1
(ZO-1)) were assessed. Oral administration of PE-MP resulted in apparent jejunal histopathological
alterations; significantly decreased mucin secretion, occludin, ZO-1, and claudin-1 expression; and
significantly upregulated MLCK mRNA, IL-1 concentration, and cleaved caspase-3 expression.
Melatonin reversed these altered parameters and improved the PE-MP-induced histopathological
and ultrastructure changes. This study highlighted the PE-MP’s toxic effect on intestinal barrier
integrity and revealed the protective effect of melatonin.
The detection of MP in a variety of food products revealed that humans are ingesting MP. Nevertheless,
there is a paucity of data about their impacts, as well as their uptake, on intestinal barrier
integrity. This study examined the toxic effects of oral administration of two doses of polyethylene
microplastics (PE-MP) (3.75 or 15 mg/kg/day for 5 weeks; mean particle size: 4.0–6.0 m) on the
intestinal barrier integrity in rats. Moreover, the effect of melatonin treatment with MP exposure
was also assessed. The PE-MP particle uptake, histopathological changes, Alcian blue staining,
Muc2 mRNA, proinflammatory cytokines (IL-1 and TNF- ), and cleaved caspase-3, as well as tight
junction proteins (claudin-1, myosin light-chain kinase (MLCK), occludin, and zonula occludens-1
(ZO-1)) were assessed. Oral administration of PE-MP resulted in apparent jejunal histopathological
alterations; significantly decreased mucin secretion, occludin, ZO-1, and claudin-1 expression; and
significantly upregulated MLCK mRNA, IL-1 concentration, and cleaved caspase-3 expression.
Melatonin reversed these altered parameters and improved the PE-MP-induced histopathological
and ultrastructure changes. This study highlighted the PE-MP’s toxic effect on intestinal barrier
integrity and revealed the protective effect of melatonin.