The present study was performed to investigate the effect of electromagnetic field (EMF) on some biochemical and hematological parameters in rats. Thirty white male albino rats 12-16 weeks old divided into two groups (15 each): the first group used as control and the second was the experimental group kept in specific test cell (this cell was designed specially for use in that experiment). Rats were exposed to the electromagnetic field for about 18 hr every day only for 3 months. Blood samples were collected from all rats after 1, 2, and 3 months from exposure to EMF in the morning from medial canthus of eyes. Samples were collected on tubes containing EDTA and divided into two parts. One part used for separation of plasma and RBCs. Plasma was used for determination of creatinine phosphokinase (CK), lactate dehydrogenase (LDH), monoamino oxidase (MAO) and acetyl cholinesterase (Ach E), as well as cortisone hormone, malondialdhyde (MDA), nitric oxide (NO) and haptoglobin were also determined. While RBCs used in preparation of hemolysate which was used for estimation of antioxidant enzymes activities. The other part still as whole blood for hematological examination. Experimental group showed alteration in serum enzymatic activities as compared to control. Moreover, increased in the serum level of MDA and NO was detected which reflected changes in the activity of biological system.