This research was undertaken in order to determine the impact of nutritional vitamin E on
genetic expression of Interferon-gamma (IFN-γ) and Interleukin-1β (IL-1β) as proinflammatory
and Interleukin-4 (IL-4) and Interleukin-10 (IL-10) as anti-inflammatory
cytokines in spleen and liver, hemagglutination inhibition antibody titers (HI titers) against
Newcastle Disease Virus (NDV), total protein, albumin, globulin, and growth efficiency of
broiler chickens. Seventy-two one-day-old Cobb broiler chicks had been allocated randomly
into two groups. Each group has 3 replicates, each with 12 chicks. The first group was given
basal diet (Control). The second group was fed the basal diet with vitamin E (100 mg/kg
diet). The obtained results revealed that dietary supplementation of vitamin E significantly
(P< 0.05) increased growth efficiency, total protein, albumin, globulin, and the antibody titers
to NDV compared to the control. Dietary supplementation of vitamin E significantly (P<
0.05) decreased the expression of pro-inflammatory (IL-1β, IFN-γ) and anti-inflammatory
(IL-4 and IL-10) cytokines in spleen and liver. In Conclusions: vitamin E supplementation
(100 mg/kg diet) can enhance growth efficiency, serum total protein, albumin, globulin, and
humoral immunity, down-regulate pro-inflammatory and anti-inflammatory cytokines gene
expression in broiler chickens.