أعضاء هيئة التدريس - جامعة بنها
Histological and immunohistochemical study of the potential therapeutic impacts of bone marrow mesenchymal stem cells and exosomes for sciatic nerve crush injury model in rats
• 2018
معلومات البحث
المؤلفون
Nahla El-Eraky El-Azaba, Abeer M. El-Mahalawaya, Ola Mostafaa and Dina Sabry b
الكلمات المفتاحية
Not Available
المجلة العلمية
Not Available
الناشر
Not Available
المجلد
Not Available
العدد
Not Available
الصفحات
Not Available
publication.type
International
رابط البحث
Not Available
المواد المرفقة
Not Available
الملخص
The aim was to evaluate the potential effect of bone marrow-derived mesenchymal stem cells
(BMSC) and BMSC-EX in the sciatic nerve injury. Forty-five rats were divided into four groups, i.e.
Group I control no surgery or treatment, Group II sciatic nerve crush surgery and 1 week postsurgery,
Group III sciatic nerve crush injury was treated with BMSC, and Group IV sciatic nerve
crush injury was treated with BMSC-EX. All animal groups were euthanized 2 weeks after
treatment. Sciatic nerves were examined histologically and immunohistochemically. Group II
showed various histological changes, i.e. vacuolation, degeneration and loss of nerve fibers.
Group II showed a significant increase in collagen fibers, but with significant reduction in osmium
tetroxide (OsO4)-stained myelin sheaths and neurofilament immunostaining compared to Group
I. Group II also revealed distortion and disruption of myelinated nerve fibers. Groups III and IV
showed significant improvement of both histological and immunohistochemical changes in
Group II. BMSC and BMSC-EX were shown to improve axonal regeneration.
(BMSC) and BMSC-EX in the sciatic nerve injury. Forty-five rats were divided into four groups, i.e.
Group I control no surgery or treatment, Group II sciatic nerve crush surgery and 1 week postsurgery,
Group III sciatic nerve crush injury was treated with BMSC, and Group IV sciatic nerve
crush injury was treated with BMSC-EX. All animal groups were euthanized 2 weeks after
treatment. Sciatic nerves were examined histologically and immunohistochemically. Group II
showed various histological changes, i.e. vacuolation, degeneration and loss of nerve fibers.
Group II showed a significant increase in collagen fibers, but with significant reduction in osmium
tetroxide (OsO4)-stained myelin sheaths and neurofilament immunostaining compared to Group
I. Group II also revealed distortion and disruption of myelinated nerve fibers. Groups III and IV
showed significant improvement of both histological and immunohistochemical changes in
Group II. BMSC and BMSC-EX were shown to improve axonal regeneration.