Tomato yellow leaf curl virus (TYLCV) is devastating to tomato (Solanum lycopersicum) crops and resistant cultivars are highly effective in controlling the disease. Breeding programmes aimed at producing TYLCV-resistant tomato cultivars have utilized resistance sources derived from wild tomato species. So far, all reported breeding programmes have introgressed TYLCV resistance from a single wild tomato source. Here, we tested degree of resistant different nine tomato species to TYLCV.Results showed that S. chilense, S. peruvianum, S. hirsutum and S.minutum appeared resistant to TYLCV. On the other hand, the maximum PPO and POD isozyme activities were recorded in the susceptible plants of S. penneleii (15) unique isoforms, while the minimum in the resistant plants of S. minutum (one). Random amplified polymorphism DNApolymerase chain reaction (RAPD-PCR) using five primers revealed the differences between the TYLCV resistant wild tomato species and the healthy control, thus the TYLCV resistant S. hirsutum revealed the highest number with 14 unique markers, followed by S. cheesmaniae with 11markers, while S. chilense and S. peruvianum revealed six markers. These polymorphic fragments are valuable for future use in tomato breeding programs, as marker assisted selection by transfer TYLCV-tolerance genes from a wild green-fruited tomato species to a cultivated line.