Phytophthora cactorum (Leb. & Cohn.) Schroet. Botrytis cinerea Pers. ex. Fr. Rhizoctonia (Corticium) solani (Prill. and Delacr) Bourd. & Galz. and Rhizopus nigricans Ehrenb. ex. Fr. were isolated from diseased fruits and plants of four strawberry cultivars namely; Fresno, Tioga, Aliso and Balady. Phytophthora cactorum was the most virulent pathogen causing most of the damage to rotted fruits. Strawberry cultivars differed as regards their resistance to infection and could be arranged as descendingly as Aliso, Freano, Tioga and Balady. Phytophthora cactorum had a wide host range in Egypt, ARE. i.e., Tomato, Potato tuber, Navel orange, Lemon and Apple. The optimum temperature, of mycelial growth ranged between 22-25°C and the best medium was Carrot Dextrose Agar (CDA) and V-8 Juice for sporulation. Fruit contact with the soil and soil moisture content were mainly responsible for fruit rot.