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Detection of Xanthomonas vesicatoria phages in infected tomato plants. 3rd Inter. Conf. Virol., Cairo Univ. Center, Nov. 24-25, 2010 Egyptian J. Virol., SP. Issue, 425-433, 2010

Egyptian J. Virol., • 2010
العودة
معلومات البحث
المؤلفون Eisa, Nawal A.; Abd EL-Ghafar, N.Y.; Abd EL-Mageed, M.H.; Mohamed, F.G. and Hasan, Eman O.
الكلمات المفتاحية Phage, Xanthomonas vesicatoria, Tomato, Bacterial spot disease, Enrichment technique
المجلة العلمية Egyptian J. Virol.,
الناشر Not Available
المجلد Not Available
العدد SP
الصفحات 425-433
publication.type International
رابط البحث Not Available
المواد المرفقة Not Available
الملخص
Different Phages parasitizing Xanthomonas vesicatoria (the causal agent of the bacterial spot disease of tomato) were isolated from infected leaves of tomato and from tomato rhizosphere soil, using enrichment technique. The phages produced plaques (4-5mm, diameter) with a distinct translucent spreading halo. Presumptive phage particles associated with X. vesicatoria were observed by Transmission Electron Microscope (TEM). Particle size and morphology of each phage isolate were examined by electron microscopy. The obtained results indicated that, the isolated phages were of the head and tail types. Four phages were detected and designated A,B,C and D, their head diameters were found to be 57.83, 43.84, 63.43, and 79.25 nm, respectively. Phages A and B were isolated from tomato leaves, whereas, phages C and D were isolated from tomato rhizosphere soils. This study seems to be the first record for phages of Xanthomonas vesicatoria under the Egyptian conditions.