Non-alcoholic fatty liver disease (NAFLD) is one of the most common causes of chronic liver disease in the world, according to the World Health Organization. In terms of cardiovascular disease, NAFLD represent a risk factor. One of the best biomarkers of platelet activation and function is the mean platelet volume (MPV). MPV has been shown to be a risk factor for atherothrombosis and a predictor of cardiovascular disease. Mean platelet volume (MPV) has been shown to be a useful tool in assessing NAFLD patients' prognosis. Methods: After metabolic syndrome was ruled out, a prospective case-control study with 25 NAFLD patients and 25 healthy controls of the same age group was carried out. Before patients were enrolled, they signed an informed consent form. The research was given the go light by the Banha University Faculty of Medicine's Ethics Committee. Results: NAFLD patients had a considerably higher MPV than the control group. Conclusion: NAFLD is a risk factor for atherosclerosis and cardiovascular disease, according to the findings of this study.