Background, nursing motivation is a critical aspect at the workplace which improves the nurses' performance in the hospital; to maintain effectiveness and development, the head nurses need to adopt with clear and attainable motivational system in the health care sector. The study was conducted at Benha University Hospital in all critical care units. The study aimed to assess the effect of educational program about motivation for head nurses on staff nurses performance. The study sample was composed of a convenient sample of 33 head nurses, and 52 staff nurses were chosen randomly. Four different tools were used in the present study for data collection, personal data questionnaire, motivation knowledge questionnaire, motivation skills observational checklist and nurses' performance observational checklist. The result showed: There was improvement in level of knowledge to more than two thirds (72.7%& 63.6%) at post program and follow up study phases, and there was a highly statistically significant improvement in level of head nurses skills about motivation nearly two third (63.6%) of head nurses had adequate motivation skills at post program. Also there was a highly statistically significant improvement in level of staff nurses performance 92.3% of them had a competent performance level at post program phase. The study concluded that there was a highly positive statistical significant correlation between head nurses' motivation knowledge, skills and staff nurses' performance at post and follow up program. The study recommended; In-service training and education programs must be conducted for refreshing and increasing knowledge and skills about the nurses' motivation art as well as to emphasize motivation skills of head nurses.