To assess pregnant women' perception regarding urinary tract infection.Material and Methods: The descriptive research design was utilized. The study was conducted in the antenatal outpatient clinic at Benha University Hospital, Egypt. A convenient sample of 105 pregnant women diagnosed with urinary tract infection. Two tools were used for data collection; 1) structured interview questionnaire which consisted of three parts; socio-demographic characteristics of the sample, obstetric data and knowledge of pregnant women regarding urinary tract infection, and 2) pregnant women' attitude towards urinary tract infection. Results: the finding illustrated that 69.5% had unsatisfactory knowledge about urinary tract infection. Meanwhile, 30.5% had satisfactory knowledge about urinary tract infection. More than two thirds 67.6% had negative attitude towards urinary tract infection. There was highly positive correlation between of knowledge and attitude regarding urinary tract infection. Conclusion and Recommendation: Two thirds of the studied women had unsatisfactory knowledge and negative attitude regarding urinary tract infection. The study recommended that raising pregnant women perception regarding urinary tract infection during antenatal visits through educational program.