For the first time in the world using the usual technique for isolating plant
fungal pathogens, successful growth and sporulation of Uromyces fabae (Pers.) De
Bary, the causal of broad bean rust, was achieved axenically on different dilutions
(strengths) of a modified MURASHIGE & SKOOG’s (MS-) tissue culture medium.
The linear growth of Uromyces fabae was more pronounced on media containing
pollen grains only and/or inositol. Regardless media strength, it was found that the
MS- medium containing pollen grains at the rate of 10 g/l. was the best for
producing uredospores. However, the total number of uredospores was reduced
proportionally by increasing dilution of media strength. Few teleutospores were also
observed on all tested media. Effect of the tested medium and its components on
characters of the resultant uredospores was also included. Under conditions of the
present work, uredospores of Uromyces fabae from axenic cultures on MS-medium
could not germinate on surfaces of inoculated broad bean leaves and reasonably
completely failed to cause any symptoms of rust disease.