Vesicular arbuscular Mycorrhizal (VAM) fungi are an important group of soil fungi because they stimulate plant growth in soils with low levels of available phosphorus, and thus improve plantlet growth and nutrition. The symbiotic status is very important for plant mineral nutrition and health. For the first time in Egypt under local conditions this study successes in developing VAM fungi (Glomus Mosseae, and G. macrocarpum) in two types of synthetic media modified M & S. However, in this media all spores vesicles, arbuscules and mycelium were obtained after one week on Perti dishes which were incubated at 35- 40°C.