The aim of present study was to investigate hematological, biochemical and oxidative state parameters in cattle spontaneously infected with bovine leukemia virus (BLV). A total 500 cattle were examined for BLV infection by enzyme linked immunosorbent assay (ELISA). Eighty (16%) animals were positive for BLV infection. Biochemical and oxidative stress markers revealed significant increases in liver enzymes Alanine Transaminase, Aspartate Transaminase and Alkaline Phosphatase (ALT, AST and ALP) activities, creatinine level and superoxide dismutase (SOD) activity associated with a significant decrease in calcium level in seropositive cattle in comparison with seronegative cattle. Meanwhile, non-significant changes were reported in levels of malondialdehyde (MDA), Nitric oxide (NO), reduced glutathione (GSH) and hematological parameters in seropositive cattle in comparison with seronegative cattle.