Stress has been defined as a barrier to concentration, problem solving, decision making, and other necessary abilities for students’ learning; it also has some symptoms and illnesses in the students such as depression and anxiety. The aim of the study was to evaluate the effect of health education program on depression, anxiety and stress among female nursing students at Benha University. A study design: A quasi experimental design was utilized in this study. Sample: A convenience random sample of 100 female students in the second and third year faculty of nursing at Benha University were selected for the intervention and control groups. Setting: The study was conducted at faculty of nursing Benha University from 2013 to 2014 .Tools: two tools were used for data collection, First an interview questionnaire to assess the socio-demography characteristics studied subjects Second :Depression, Anxiety and Stress Scale (DASS-42).The study results: revealed that improvement in reducing mean scores of depression(7.1), anxiety (7.62) and stress (11.68) in the study group post immediate & three months after program. The study concluded that effect of health educational program among female nursing students had positive effect in reducing stress, anxiety and depression in the study group post immediate & three months after program. The study finding recommended: holding stress management workshops to improve mental health of the students.