Objective: Comparing different effects of supplementation of Ham’s F10 media with
vitamin E, Baker’s Yeast (Saccharomyces cerevisiae) extraction and different
concentrations of vitamin C on sperm parameters.
Patients and methods: Forty samples of ejaculated semen from infertile males were collected. Six
equal fractions of each semen sample were created. A Control group (activated with Ham’s F10 only). 5
study groups were activated with Ham’s F10 plus: Group 1:Vit E (conc. 2 mg/ml Ham’s F10), groups 2,3,4
(Vit C1, Vit C2 and Vit C3) (conc.0.02, 0.04, 0.06 mg/ml Ham’s F10, respectively) ), Group 6: Yeast ( 20
mg/ml Ham’sF10).
Results: The treated groups showed considerable improvements over the control
group in vitality, total motility, progressive motility, and oxidative stress.
Comparable results were found between the Vit. E group (72%, 45%, 95%& 0) and
the Yeast group (66%, 39%, 95%& 0), and the best result for Vit. C was with conc.
(0.06 mg/ml) in group Vit. C3(65%,39%, 95%& 0).
Conclusions: Vitamin C, vitamin E, and yeast all have positive effects on semen
parameters. Vit.E has the best results than Vit. C and yeast. The best concentration of
Vit. C to achieveresults is 0.06 mg/ml Ham’s F10.