Staphylococcus aureus causes a wide range of diseases in human beings, from minor skin infections to severe illnesses such as septicemia, toxic shock, endocarditis, and pneumonia . Staphylococcus aureus is a major pathogen of increasing importance due to the rise in antibiotic resistance. S. aureus was an important cause of disease in poultry it could be involved in a wide range of clinical conditions such as septicemia, bone and Joint infections, abscesses and dermatitis.In this study , 160 samples were collected , out of which 80 samples were from poultry and human patients (80 for each ). The incidence of infection with S.aureus in Bumble foot, Skin swabs in chicken and Diabetic foot swab , Skin sawb and Nasal swab in human were , 10% , 66% , 30% ,40%,67.5% respectively. Methicillin resistant S.aureus (MRSA) were incidence in chicken and human 66.6% and 33.3% respectively. Subjected to PCR for detection of some antimicrobial resistance genes using intrinsic methicillin resistant gene (mec A) ,the IgG binding region of protein A( spa) gene were 83.3% and 50 % respectively also beta-lactamase (blaZ) gene 100% and 100% respectively were detected in most MRSA isolates either from human or chicken samples.