In the present study antimicrobial activity of methanol Cuminum cyminum extract (50mg/ml) in combination with some antibiotics were investigated against Staphylococcus aureus isolates. The antimicrobial sensitivity pattern of these isolates in vitro was done and the intermediate isolates were checked for combination with methanol Cuminum cyminum extract after detecting their antimicrobial activities against these isolates by both disc diffusion test and MIC and the results showed that the antimicrobial activities of rifampicin, enrofloxacin, doxycycline, gentamicin, and erythromycin were enhanced in combination with methanol Cuminum cyminum extract the obtained data showed that enhancement by both disc diffusion test and MIC for methanol Cuminum cyminum extract and each of mentioned antibiotics in decimal assay of additivity ratio (0.5 for rifampicin and erythromycin and 0.5 for extract ,0.7 for enrofloxacin, and 0.3 for extract,0.6 for doxycycline, gentamicin and 0.4 for extract) against S.aureus isolates .