The raw Jojoba oil is utilized to produce biodiesel via a transesterification process, which is optimized using smoke point, yield, and viscosity of produced biodiesel, as well as the final product, is characterized utilizing GC-MS and FTIR. Then, the combustion and emission characteristics of Jojoba oil biodiesel-Jet-A1 blends are performed utilizing a lean premixed pre-vaporized combustion (LPP) approach. The JME with two blending ratios of 10% and 20% by volume and jet fuel of 90% and 80% by volume, as well as the pure jet fuel, are burned in an LPP combustor at two different equivalence ratios of 0.87 and 0.95 to investigate the suitability of using the JME-jet A1 fuel blends as an alternative energy resource. The LPP combustor is designed precisely regarding a comprehensive survey of the main parameters affecting on it, and its combustion results show that it has an acceptable and stable flame. The results indicate that the NOx emission decreases with enlarging the volume fraction of JME, while the CO and UHC formation increase. The flame temperature distributions are similar for the different tested fuels. It can be deduced that Jojoba biodiesel can be used up to 20% by volume as an alternative fuel with jet fuel in a commercial application.