Meat and bone meal (MBM) is considered an organic waste with abundant nutrient elements. There is an urgent demand for new technologies to recycle MBM. MBM treatment with black soldier fly larvae (BSFL) is of greater potential to obtain more available C and N than traditional methods. This study evaluated the effect of batch feeding times on MBM bioconversion by BSFL, and investigated the emission of greenhouse gases and NH3, the final distribution of C and N during this treatment. Our results showed that the lowest greenhouse gases and NH3 emission was observed in 1-time batch feeding treatment. The total greenhouse gases were increased with the increasing batch feeding times, the highest emission (484.13 g CO2-eq/kg DM) was obtained in the 5-time batch feeding treatment. The 5-time batch feeding treatment also achieved the highest substrate conversion efficiency (31.17%). Overall, using MBM as substrate for BSFL batch feeding is considered a very promising alternative.