This work was conducted in the Apiary of Plant Protection Research Institute at El-Qanater El-Khiriya to study the effect of additive pollen substitute feeding of honey bee colonies on brood rearing, queen’s egg laying, pollen gathering and honey production through the period of 1st March to 29th August 2012. Results revealed that feeding the honey bee colonies on additive pollen substitute and sugar syrup (1:1) make honey bee colonies have significantly more brood rearing, queen's egg laying, pollen gathering and honey production than the control colonies which fed on sugar syrup only.