Objective : to determine the association of human leucocytic antigen (HLA) class I and class II with juvenile rheumatoid arthritis in Egyptian children and its relation to clinical and laboratory findings. Methods : Fifity Egyptian children patients with JRA and 40 controls were included in this study, they were subjected for complete clinical examination and laboratory investigations. Rheumatoid factor (RF) was performed using latex test while antinuclear antibodies (ANA) were estimated by ELSA. For HLA-class I typing . Lympho-microcytotoxicity technique was perfrormed, while for HLA class II typing . PCR-SSOP using innolipa was used . Results : there was significant positive association of JRA pattients with HLA class I , HLA -B (B13 , B27) and class II HLA DR B1 alleles (*1010, 0102 , *0801 and * 1104) HLA -DQA1 alleles ( *0401) and HLADQB1 alleles (*0202 , *0303 *0603 -09) . While HLA - B12 and HLA DQB1 *0602 were significntly positive associated with healthy controls, also there was positive association between HLA.DQA1 *0501 and HLA-DQB1 *0603 and pauciarticular onset JRA while polyarticular type was positive associated HLADQA1 *0101 A significant positive association was found between ANA positive patients and HLA-DRB1 * 1104 allele.