A total number of 9 Campylobacter isolates were collected from different farm animals (cattle, buffaloes, sheep and camels). All strains were identified biochemically. Outer membrane protein (OMP) of each isolate was extracted and then subjected to Sodium dodecyle sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) for determination of molecular weight (MW) of each protein component. The biochemical identification of the nine isolates revealed that, three isolates were belonged to C. fetus subsp. venerealis, two isolates were C. fetus. subsp. fetus, two isolates were C. jejuni and two isolates were C. coli. The electrophoretic patterns of Campylobacter OMPs showed (6-11 bands) with different MWs ranging from 219 to 14 kDa. Seven strains had a common band with molecular weight of 97.633 KDa, six strains had 84.703 kDa, five strains expressed 157.325, 155.91 & 75.632 kDa, four strains expressed kDa with 111.55, 107.38, 54.24, 47.418, 41.375& 27.348, three strains expressed kDa with 175.57, 111.19, 109.43, 105.115, 102.575, 63.795, 34.579 & 22.1165, two strains expressed KDa with 219, 213.805, 165.795, 71.671 & 17.882 and only one strain expressed kDa with 205, 162.92, & 15.432. From the obtained results of the present study, it can be concluded that, each Campylobacter species has a distinct protein pattern which can be used to distinguish between different strains. Protein bands with MW of 97.633 and 84.703 kDa showed to have immunogenic activity. SDS-PAGE can be considered as a rapid, reliable, sensitive and diagnostic tool for characterization of campylobacter isolates.