The present investigation aimed to assess the sperm metabolism in rabbits. Six mature NZW bucks were used. Ejaculates were extended in sodium citrate buffer and incubated at 37 degrees Celsius for 3 hours. Significant decrease in the number of motile
and viable sperm cells was noticed with the advancement of incubation. The time required for reduction of methylene blue was prolongued during the second and third hours of incubation.