Johne’s disease is chronic enteric incurable disease in ruminant, is caused by Mycobacterium avium subsp.paratuberculosis (MAP). The data about incidence and economic importance of the disease in Egypt are scare. Thepresent study investigated the prevalence of Johne`s disease among cattle in some localities in Egypt. The resultsrevealed that the infection was significantly higher in older animals than young animals (P=