Type I diabetes is considered to be an autoimmune disorder which occurs when the immune system attacks the body’s own tissues and organs. In the present study, patients were divided into four groups according to their health state with five patients each. In addition to a normal group which represents healthy individuals. Blood samples were collected from each individual in the five groups to determine blood glucose, serum total bilirubin, direct bilirubin, albumin, total protein, ALT, AST, urea and uric acid were measured in each sample. The results showed that all biochemical parameters were around the normal levels. Whereas, the HLA-DQA1 exon2 gene of the four studied groups were mutated when compared to the normal and NCBI reference.