A green-house experiment was conducted to investigate the efficiency of three heavy metal-tolerant plant growth promoting bacterial strains (HMT-PGPB) (Alcaligenes faecalis MG257493.1, Bacillus cereus MG257494.1 and Alcaligenes faecalis MG966440.1 to alleviate the heavy metal's toxic effects on sorghum plant (Sorghum bicolor, L.) in addition to their ability to enhance plant growth. The oxidative enzymes, photosynthetic pigments, growth characteristics, heavy metals uptake and heavy metals translocation factor (TF) were estimated in sorghum cultivated in soil contaminated with heavy metals under green-house conditions. Results showed that the application of these HTM-PGPB strains as biofertilizer of sorghum help plant to ignore the toxic effects of heavy metals and enhance growth characteristics.